Supervisor: Mario Hollomon SB 304
Dr. Mario Hollomon’s laboratory investigates the effect of autophagy on cancer development and metastasis. Specifically, the laboratory investigates the effect of autophagy modulation on the sensitivity of cancer cells to anticancer therapy. Using lenitvirus, we have generated several stable knockdown cancer cell lines that have enabled the laboratory to investigate the effect of autophagy proteins on the response of cancer cells to anticancer drug treatment. We have shown that autophagy inhibition, through knockdown of ATG5, can have an opposing effect on osteosarcoma cells treated with camptothecin. We are currently investigating the mechanisms responsible for this opposing effect. We are also investigating the effect of other autophagy-related proteins on the response of cancer cells to anticancer drug treatment.
The laboratory also investigates cell death signaling pathways in cancer cells. Here, we also use lentivirus to knockdown the expression of select cell death pathway-associated proteins to better understand their role in the cell death pathway. In addition, the laboratory also investigates the role of endogenous antioxidants in protecting cancer cells against anticancer drug-induced cytotoxicity.